Microbiological assays

Determination of biologically active substances using microorganisms

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Microbiological assays

The use of microorganisms to determine biologically-active substances is tried and tested. In doing so, it is possible to reliably detect B-vitamins and antibiotics.

Microorganisms only grow under certain conditions: some bacteria, for example, require a specific pH-value, a specific temperature range and a specific nutrient in order to grow. These specific characteristics of microorganisms can be taken advantage of in the analysis.

VitaFast┬« ÔÇô microbiological microtiter plate to determine the vitamin concentration

The microbiological microtiter plates for the VitaFast┬« product line are easy tests that can be used to determine the vitamin content in food, drinks, animal feed and pharmaceutical products.  The vitamin is extracted from the sample material for the verification. The extract is diluted and together with the assay medium, it is mixed in the cavities of the microtitre plates that are coated with a specific microorganism. If the vitamin in question is present in the sample, this microorganism will continue to grow until the vitamin is completed used up. After an incubation period, the microbiological growth is measured as turbidity and compared with a standard concentration series. The measurement takes place in a microtiter plate photometer.

Premi┬«Test ÔÇô a microbial rapid screening test for the detection of antibiotics

The Premi®Test is a microbial screening test for the detection of antibiotic residues in food and animal feed in less than four hours. The test enables a fast and easy screening of meat (beef, pork, poultry), fish, prawns, eggs, liver, kidney, urine and animal feed to check for the residues of β-lactam, cephalosporin, macrolide, tetracycline, sulfonamide, aminoglycoside, quinolone, amphenicol and polypeptide antibiotics and can be used during the entire manufacturing chain by slaughterhouses to meat processing companies through to analytical laboratories.

The test is based on the sensitivity of the thermophilic bacteria, Bacillus stearothermopilus for antimicrobial substances. A defined number of spores are embedded into the nutrient agar in ampules. If a sample is tested, which is free of antibiotic residues, the spores germinate during the three-hour incubation phase and produce an acid during the following growth phase. This leads to a drop in the pH-value and there is a change in colorÔÇöfrom the original violet to yellowÔÇö of the pH-indicator bromocresol found in the agar. However, if a sample is tested that does contain antibiotic residues, the germination of the spores is inhibited and the ampules violet color does not change after incubation.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
Premi®Test Urin

Premi®Test Urin is a reagent that enables bovine/porcine urine to be tested for antibiotic residues with Premi®Test.

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Premi®Test Urine contains 1 dropping bottle with 15 ml of sample buffer. The number of samples which can be prepared is dependent on the volume of the samples. Exemplarily, 30 samples with a volume of 30 ml each. R3921
Premi®Test 25

Premi┬«Test is a microbial screening test that allows the detection of antibiotic residues, especially in fresh meat (beef, pork, poultry) in less than 4 hours. The test is sensitive to antibiotics of the following groups: ╬▓-lactams, cephalosporins, …

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25 ampoules R3925
Premi┬«Test 4×25

Premi┬«Test is a microbial screening test that allows the detection of antibiotic residues, especially in fresh meat (beef, pork, poultry) in less than 4 hours. The test is sensitive to antibiotics of the following groups: ╬▓-lactams, cephalosporins, …

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4 x 25 ampoules R3900
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ProductDescriptionNo. of tests/amountArt. No.
VitaFast® Vitamin C (L-Ascorbic Acid)

VitaFast┬« Vitamin C (L-Ascorbic Acid) is a test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in foods, pharmaceutical products and other sample material. Furthermore the total amount of vitamin C …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1010
VitaFast® Folic Acid

The VitaFast┬« Folic Acid microtiter plate test is a microbiological method for the quantitative determination of total folic acid (added and natural folic acid) in food, animal feed and in pharmaceutical products. The microbiological test system is …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1001
VitaFast® Vitamin B7 (Biotin)

The VitaFast┬« Biotin microtiter plate test is a microbiological method for the quantitative determination of total biotin (added and natural biotin) in food and pharmaceutical products. The microbiological test system is in accordance with …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1003
VitaFast® Vitamin B6 (Pyridoxin)

The VitaFast┬« Vitamin B6 (Pyridoxin) microtiter plate test is a microbiological method for the quantitative determination of total vitamin B6 (added and natural vitamin B6) in food, animal feed and in pharmaceutical products. The microbiological …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1008
VitaFast® Pantothenic Acid

The VitaFast┬« Pantothenic Acid microtiter plate test is a microbiological method for the quantitative determination of total pantothenic acid (added and natural pantothenic acid) in food, animal feed and in pharmaceutical products. The …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1005
VitaFast® Vitamin B3 (Niacin)

The VitaFast┬« Niacin microtiter plate test is a microbiological method for the quantitative determination of total niacin (added and natural niacin) in food, animal feed and in pharmaceutical products. The microbiological test system is in …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1004
VitaFast® Vitamin B2 (Riboflavin)

The VitaFast┬« Vitamin B2 (Riboflavin) microtiter plate test is a microbiological method for the quantitative determination of total vitamin B2 (added and natural vitamin B2) in food, animal feed and in pharmaceutical products. The microbiological …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1007
VitaFast® Vitamin B12 (Cyanocobalamin)

The VitaFast┬« Vitamin B12 (Cyanocobalamin) microtiter plate test is a microbiological method for the quantitative determination of total vitamin B12 (added and natural vitamin B12) in food and in pharmaceutical products. The microbiological test …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1002
VitaFast® Vitamin B1 (Thiamin)

The VitaFast┬« Vitamin B1 (Thiamine) microtiter plate test is a microbiological method for the quantitative determination of total vitamin B1 (added and natural vitamin B1) in food, animal feed and in pharmaceutical products. The microbiological test …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1006
VitaFast® Inositol

The VitaFast┬« Inositol microtiter plate test is a microbiological method for the quantitative determination of total inositol (added and native inositol) in food, animal feed and in pharmaceutical products. The microbiological test system is in …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) P1009
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