ELISA

Efficient immunoassays for food analysis

Technologies

ELISA

The enzyme-linked immunosorbent assay (ELISA) is an antibody-based test method. This widespread technology is sensitive, fast and reliable.

With their ELISA test kits, R-Biopharm relies on a well-established analytical method that meets the requirements of modern laboratories. RIDASCREEN® tests show a high sensitivity and are characterized by robustness. For the time-sensitive screening of various commodities, RIDASCREEN®FAST tests are offered. These tests enable to analyze food and feed samples within a maximum of 30 min including sample preparation.

ELISA principle

Antibodies are proteins produced in plasma cells of vertebrates as part of the adaptive immune system against structures (antigens) which are recognized as foreign to the body. Antibodies bind to their antigens by a distinct pattern of ionic and hydrophobic interactions, hydrogen bridge linkages and Van-der Waals forces. The interaction between antibody and its antigen is selective and highly specific, similar to a lock and key.

The enzyme-linked immunosorbent assay is based on this selective and specific antibody-antigen recognition. Many formats of qualitative or quantitative ELISA tests have been established. Performing an ELISA test involves at least one specific antibody for a particular antigen. A main principle is that one of these immunological components is immobilized to a solid phase, the cavities of a microtiter plate. The analyte from the sample interacts with the antibody-antigen system. This interaction can be visualized by enzymes, linked to secondary antibodies or antigens, and indicate if an antibody-antigen binding has occurred. An added substrate is converted by the coupled enzyme resulting in a change of colour, which can be measured with a spectrophotometer.

Sandwich ELISA and competitive ELISA

While a sandwich ELISA detects only intact proteins and large fragments with at least two binding sites, a competitive ELISA is able to detect large and small protein fragments with only one binding site. For this reason, the competitive ELISA can also be used for highly processed food products.

Good ELISA practice

The manual provides a comprehensive overview for both – beginners and advanced analysts, in order to improve the quality of performed ELISA analysis. It represents a guideline which will help you to establish reasonable framework conditions and conditions of use, which shall be complied with to achieve the best possible performance when using R-Biopharm ELISA test kits.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Histamine (enzymatic)

RIDASCREEN® Histamine (enzymatic) is used for the quantitative determination of histamine in fresh fish, canned fish, fish meal, wine and cheese.

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Microtiterplate with 96 wells (12 strips with 8 removable wells each) R1605
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Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Total Gluten

RIDASCREEN® Total Gluten (Art. No. R7041) is a sandwich enzyme immunoassay for the quantitative determination of intact (non-hydrolyzed) gluten from gluten containing cereals (wheat, rye and barley) in oat and oat products.

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7041
RIDASCREEN® Peanut

RIDASCREEN® Peanut is a sandwich enzyme immunoassay developed for the quantitative analysis of peanut or peanut proteins in food. Due to the large number of different food products, the following samples were examined representatively for different …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R6811
RIDASCREEN® Egg

RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R6411
RIDASCREEN®FAST Gliadin sensitive

RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour).

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7051
RIDASCREEN® Gliadin

RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7001
RIDASCREEN®FAST Cashew

The RIDASCREEN®FAST Cashew is a sandwich enzyme immunoassay developed for the quantitative analysis of Cashew in food. Exemplarily for the food groups pastries and sweets have been tested during the assay’s development: cookies, chocolate and ice …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6872
RIDASCREEN® Histamine (enzymatic)

RIDASCREEN® Histamine (enzymatic) is used for the quantitative determination of histamine in fresh fish, canned fish, fish meal, wine and cheese.

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Microtiterplate with 96 wells (12 strips with 8 removable wells each) R1605
RIDASCREEN®FAST Milk

RIDASCREEN®FAST Milk is a sandwich enzyme immunoassay to quantify milk proteins (casein and Ăź-lactoglobulin) in food containing whey, milk or milk powder such as sausages, ice cream, chocolate, bakery goods, cake and bread mix, soups, sauces, …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R4652
RIDASCREEN® Histamin

RIDASCREEN® Histamin is a competitive enzyme immunoassay for the quantitative analysis of histamine in white and red wine, sparkling wine, milk, cheese, fresh fish, canned fish and fish meal.

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R1601
RIDASCREEN®FAST β-Lactoglobulin

RIDASCREEN®FAST β-Lactoglobulin is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed β-lactoglobulin in rice crispies, chocolate and sausage.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R4912
RIDASCREEN® Gliadin competitive

RIDASCREEN® Gliadin competitive is used for the analysis of fermented and hydrolyzed food (e.g. beer, starch syrup, starch, malt extract, sourdough, soy sauce) which are declared as “gluten-free”. The R5 monoclonal antibody recognizes …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7021
RIDASCREEN®FAST Gliadin

RIDASCREEN® FAST Gliadin is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) in raw products like flours (buckwheat, rice, corn, oats, teff) and …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R7002
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Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® SET Total (96 tests)

RIDASCREEN® SET Total is a sandwich enzyme immunoassay for the combined detection of Staphylococcus aureus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures.

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Microtiter plate with 96 wells (12 strips with 8 breakable wells each),
96 determinations.
R4105
RIDASCREEN® SET A,B,C,D,E

RIDASCREEN® SET A,B,C,D,E is a sandwich enzyme immunoassay for the identification of Staphylococcus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures. Based on its sensitivity the RIDASCREEN® SET A,B,C,D,E test …

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Microtiter plate with 96 wells (12 strips with 8 wells each),
one strip is necessary for analysis of one sample,
12 tests in total.
R4101
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Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® DON

RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R5906
RIDASCREEN®FAST Fumonisin

RIDASCREEN®FAST Fumonisin is a competitive enzyme immunoassay for the quantitative determination of fumonisin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R5602
RIDASCREEN®FAST DON SC

RIDASCREEN®FAST DON SC is a competitive enzyme immunoassay for the quantitative determination of DON in cereals, malt and feed with a sinlge control standard curve.

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Microtiter plate with 48 wells (6 strips with 8 wells each). R5905
RIDASCREEN®FAST DON

RIDASCREEN®FAST DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt and feed.

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R5901: microtiter plate with 96 wells (12 strips with 8 removable wells each)
R5902: microtiter plate with 48 wells (6 strips with 8 removable wells each)
R5901 / R5902
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Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Nitrofuran (SEM)

RIDASCREEN® Nitrofuran (SEM) (R3724) is a competitive enzyme immunoassay for the quantitative analysis of semicarbazide (SEM) in shrimp, fish, meat (bovine, porcine and chicken).

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Microtiter plate with 96 wells (12 strips with 8 wells each). R3724
RIDASCREEN® Nitrofuran (AHD)

RIDASCREEN® Nitrofuran (AHD) is a competitive enzyme immunoassay for the quantitative analysis of the nitrofurantoin metabolite AHD in shrimp and fish.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3713
RIDASCREEN® Nitrofuran (AMOZ)

RIDASCREEN® Nitrofuran (AMOZ) is a competitive enzyme immunoassay for the quantitative determination of AMOZ in shrimp, fish, meat and poultry.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3722
RIDASCREEN® Nitrofuran (AOZ)

RIDASCREEN® Nitrofuran (AOZ) is a competitive enzyme immunoassay for the quantitative analysis of AOZ in shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3703
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