SureFast® Norovirus/Hepatitis A 3plex
The SureFast® Norovirus/Hepatitis A 3plex is a multiplex real-time RT-PCR for the direct qualitative detection and differentiation of norovirus (genogroup I and II) and hepatitis A.
The test contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample preparation procedure and to monitor possible PCR-inhibition.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm (FAM, VIC and Cy5) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Applied Biosystems 7500 and Qiagen RotorGene Q.
|Test format||100 reactions|
|Sample Preparation||For DNA preparation the use of SureFast® PREP DNA/RNA Virus is recommended.|
|LOD (Detection Limit)||The SureFast® Norovirus/Hepatitis A 3plex real-time RT-PCR has a limit of detection of ≤ 25 RNA copies. The assay limit of detection depends on RNA preparation and RNA content.|