SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

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Product info

SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

Intended use

The multiplex real-time PCR test detects DNA of macadamia, brazil nut and pecan according to directive (EC) 1169/2011 qualitatively. Each reaction contains an internal amplification control (IAC).

General Information

For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.

The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. Internal validation was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent Aria Dx, Bio-Rad CFX96, Roche LightCycler® 480 II2, Roche cobas z 480 Analyzer2 and Qiagen Rotorgene Q.

Accessories

Specifications
Art. NoS3403
Test format100 reactions
LOD (Detection Limit)ÔëĄ 0.4 mg / kg
Cross Reactivity.

For the Pecan real-time PCR system cross reactivity was observed with DNA extracts from shagbark hickory (Carya Ovata).

EvaluationReal-time PCR instrument with four detection channels (510 nm, 580 nm, 610 nm and 660 nm)