RIDASCREEN® Sulfamethazin

Art. No.: R3011

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Product info

RIDASCREEN® Sulfamethazin

Art. No.: R3011

Intended use

RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg.

General Information

Sulfonamides are widely used as feed additives, mainly for fattening of calves and pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim, tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pneumonitis and other (systemic) diseases. Sulfonamide residues may therefore occur in food of animal origin such as meat and milk. Particularly the transmission of the carcinogenic sulfamethazine represents a threat to human health. The EU established a maximum residue limit (MRL) for all substances of the sulfonamide-group of 100 ╬╝g/kg (ppb) in muscle, fat, liver, kidney and milk.

 

Benefits:

  • High specificity for sulfamethazine only: No significant cross reactivity to other sulfonamides.
  • Shortened test implementation of 45 min only.
  • Improved sensitivity and recovery.
Specifications
Art. NoR3011
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each).
Sample preparationMilk: direct use, meat: homogenization, centrifugation

Honey: extraction, clean-up with RIDA® C18 column, evaporation, reconstitution, precipitation

Liver, kidney, fish: homogenization, centrifugation, precipitation

Shrimp, egg: homogenization, extraction, evaporation, reconstitution
Incubation time45 min
LOD (Detection Limit)Milk approx. 4 ╬╝g/l, meat (bovine, porcine) approx. 5 ╬╝g/kg, meat (poultry) approx. 10 ╬╝g/kg, honey approx. 10 ╬╝g/kg*, liver approx. 6 ╬╝g/kg, kidney approx. 10 ╬╝g/kg, fish approx. 7 ╬╝g/kg, shrimp approx. 15 ╬╝g/kg, egg approx. 16 ╬╝g/kg
*For honey the LOD can deviate due to matrix effects.
Detection CapabilityMilk 10 ┬Ág/l, meat (bovine, porcine) 10 ┬Ág/kg, meat (poultry) 15 ┬Ág/kg, honey 20 ┬Ág/kg, liver 15 ┬Ág/kg, kidney 15 ┬Ág/kg, fish 20 ┬Ág/kg, shrimp 20 ┬Ág/kg, egg 15 ┬Ág/kg
Validated matrices

Milk, meat (bovine, porcine), meat (poultry), honey, liver, kidney, fish, shrimp and egg.

Detected analyte

Sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg.

EvaluationMicrotiter plate spectrophotometer (450 nm)