RIDASCREEN® Risk Material
RIDASCREEN® Risk Material is a sandwich enzyme immunoassay for the quantitative analysis of risk material (CNS) in processed (heated) meat and sausages.
For the determination of risk material (CNS) in raw meat, meat products and on contaminated surfaces we refer to the RIDASCREEN® Risk Material 10/5 test with Art. No.: R6703.
BSE (bovine spongiform encephalopathy) has been diagnosed first in Great Britain in 1986. The molecular mechanisms of infection are still unresolved but it is clear, that the Prion protein (prPsc) plays a major role in the transmission and development of the brain disease. Feeding of insufficient heated animal meal is likely to be the main cause of the spread of disease which has caused more than 180000 dead cows up to the end of 2000. Application of extracts from infected brain induced infection in different animal species. Similar diseases are now described in cows, sheep, goat, cat and many other mammalians. With a high presumption, the new variant of the human Creutzfeldt-Jakob disease (vCJD) is caused by infected beef and beef products. The mortality of this disease is 100 %, with 111 deaths in Great Britain up to May 2002. As a protective measurement, animal meal / protein as animal feed is no longer allowed (2000/766/EG) and potential risk material has been banned from food production by the EU (2000/418/EG)/(2001/999/EG).
The detection of risk material by RIDASCREEN® Risk Material occurs by the determination of glial fibrillary acidic protein (GFAP), a cellulary marker which can be found very high concentrated in CNS tissue.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each). |
|Sample preparation||Homogenization, extraction, centrifugation, dilution|
|Incubation time||1 h (20 - 25 °C)|
|LOD (Detection Limit)||≤ 0.2 % for CNS tissue in processed (heated) meat and sausages|
Processed (heated) meat and sausages
|Detected analyte||Glial fibrillary acidic protein (GFAP of CNS tissue from cattle, veal, sheep, goat, horse, poultry and pig.|
|Evaluation||Microtiter plate spectrophotometer (450 nm) |