11+Myco MS-PREP®

Art. No.: RBRP128 / RBRP128B

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Product info

11+Myco MS-PREP®

Art. No.: RBRP128 / RBRP128B

Intended use

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, Deoxynivalenol, Zearalenone, T-2 and HT-2 in a wide range of commodities in conjunction with LC-MS/MS.

General Information

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.

Benefits:

  • Improve workflow using innovative product.
  • Simultaneous detection of 11 or more legislated mycotoxins.
  • Single extraction, with excellent precision and accuracy.

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Specifications
Art. NoRBRP128 / RBRP128B
Test formatRBRP128: 10 immunoaffinity columns
(3 ml format),
RBRP128B: 50 immunoaffinity columns
(3 ml format)
Sample preparationA representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on he commodity analysed), filtered and diluted ready for applying to the immunoaffinity column.
LOD (Detection Limit)Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used.

B1 0.007 ng/ml, B2 0.007 ng/ml,
G1 0.004 ng/ml, G2 0.015 ng/ml,
OTA 0.050 ng/ml, FB1 1.000 ng/ml,
FB2 0.250 ng/ml, DON 1.950 ng/ml,
ZON 1.560 ng/ml, T-2 0.049 ng/ml,
HT-2 0.195 ng/ml
Detected analyte

Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Ochratoxin A, Ochratoxin B Fumonisin B1, Fumonisin B2, Fumonisin B3 Deoxynivalenol, 3-acetyl DON, Zearalenone, ╬▒-Zearalenol, ╬▓-Zearalenol, Zearalanone, ╬▒-Zearalanol, ╬▓-Zearalanol, T-2 and HT-2.

Available application notes

Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.

EvaluationHPLC or LC-MS/MS.