VitaFast® Vitamin C (L-Ascorbic Acid)

Art. No.: P1010

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Product info

VitaFast® Vitamin C (L-Ascorbic Acid)

Art. No.: P1010

Intended use

VitaFast® Vitamin C (L-Ascorbic Acid) is a test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in foods, pharmaceutical products and other sample material. Furthermore the total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid) can be detected (see application note).

General Information

Ascorbic acid is a clear, odourless, cristalline solid substance which has a sour flavour. There are four stereoisomeric variants but only L-(+)-ascorbic acid (also referred to as vitamin C) is biologically active. Its vitamin activity is the most relevant property of L-ascorbic acid: humans cannot produce vitamin C and require it as part of their nutrition. A deficiency can result in the manifestation of scurvy. L-ascorbic acid and its salt, the anion L-ascorbate, will hereinafter be referred to as ascorbic acid and ascorbate, respectively.

Benefits:

  • The method is robust and suitable for the routine determination of the ascorbic acid content in a multitude of foodstuffs and beverages.
  • This test has very low detection and quantification limit.
Specifications
Art. NoP1010
Test formatMicrotiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparationHomogenisation, extraction, filtration, dilution,
Incubation timeStep 1 = 20 min in the dark at 20-25 ┬░C,
Step 2 = 30 min in the dark at 20-25 ┬░C.
LOD (Detection Limit)7.8 mg / 100 g (ml)
LOQ (Limit of quantification

7.8 mg / 100 g (ml)

Validated matrices

Fruit gums, multivitamin effervescent tablets, Iron and vitamin C tablets.

Detected analyte

Ascorbic acid concentration

Available application notes

Total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid)

EvaluationMicrotiter plate photometer 550-570 nm