SureFood® ALLERGEN Walnut

S3607

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Analytes

Product info

Intended use

The SureFood® ALLERGEN Walnut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific walnut (Juglans regia and Juglans nigra) DNA sequences according to directive (EC) 1169/2011.

General Information

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg walnut / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.

The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.

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Specifications
Art. NoS3607
Test format100 reactions
LOD (Detection Limit)ÔëĄ 0.4 mg / kg
LOQ (Limit of quantification

1 mg / kg using SureFood® PREP Advanced, protocol 1

EvaluationReal-time PCR instrument with two detection channels (510 nm and 580 nm)
E-Mail linksales@r-biopharm.de