SureFood® ALLERGEN Fish

Art. No.: S3610

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Product info

SureFood® ALLERGEN Fish

Art. No.: S3610

Intended use

The real-time PCR test detects DNA of fish according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

General Information

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg fish / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, AriaDx and Agilent Mx3005P.

The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.

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Specifications
Art. NoS3610
Test format100 reactions
LOD (Detection Limit)ÔëĄ 1 mg / kg
LOQ (Limit of quantification

4 mg / kg using SureFood® PREP Advanced, protocol 1

Cross Reactivity.

Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata).

EvaluationReal-time PCR instrument with two detection channels (510 nm and 580 nm)