SureFast® Enterobacteriaceae Screening PLUS

F5507

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Analytes

Product info

Intended use

The test detects Enterobacteriaceae DNA.

Each reaction contains an internal amplification control (IAC).

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.

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Specifications
Art. NoF5507
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection Limit)The SureFast® Enterobacteriaceae PLUS real-time PCR has a limit of detection of 5 DNA copies.
Cross Reactivity.

Cross reactivity was observed with DNA extracts from Aeromonas species and Aggregatibacter aphrophilus.

Files
InstructionsGerman/English