RIDASCREEN®FAST Lysozym

Art. No.: R6452

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Analytes

Product info

RIDASCREEN®FAST Lysozym

Art. No.: R6452

Intended use

RIDASCREEN┬«FAST Lysozym is a sandwich enzyme immunoassay for the quantitative analysis of lysozyme (henÔÇÖs egg protein) in food like wine, cheese and sausage.

General Information

HenÔÇÖs egg (Gallus gallus) is very rich of proteins and represents an important food source for humans. While proteins of egg yolk only have minor allergenicity, many proteins of egg white are known to be allergenic. Lysozyme is one of the allergenic egg white proteins and contributes with approx. 3.5 % to the egg white proteins. In addition to ovalbumin, ovomucoid and ovotransferrin even very low amounts of the lysozyme allergen can cause allergic reactions, which may lead to an anaphylactic shock in severe cases and was therefore included in allergen legislation. Due to its bacteriostatic effect, lysozyme is often used for the preservation of food products such as cheese and sausages (E1105). The allergen can also be present as an ingredient or as a contamination in raw and cooked products. According to regulation (EU) No. 1169/2011 egg and products thereof have to be declared on the food labels. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.

Benefits:

  • Fast test kit: short incubation time (30 min) compared to competitor.
Specifications
Art. NoR6452
Test formatMicrotiter plate with 48 wells (6 strips with 8 removable wells each)
Sample preparationHomogenization, extraction and centrifugation
Incubation time30 min
Room temperature
LOD (Detection Limit)'- Wine: 0.005 mg / kg (ppm) lysozyme (mean)
0.004 ÔÇô 0.006 mg / kg (ppm) lysozyme*

- Cheese, sausage: 0.011 mg / kg (ppm) lysozyme (mean)
0.006 ÔÇô 0.016 mg / kg (ppm) lysozyme*
*depending on matrix
LOQ (Limit of quantification

‘- Wine: 0.05 mg / kg (ppm) lysozyme
– Cheese, Sausage: 0.25 mg / kg (ppm) lysozyme

Validated matrices

Wine, cheese and sausage.

Detected analyte

Lysozyme

Available application notes

More sensitive allergen analysis in wine.

EvaluationMicrotiter plate spectrophotometer (450 nm)