OCHRAPREP®

Art. No.: RBRP14 / RBRP14B

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Product info

OCHRAPREP®

Art. No.: RBRP14 / RBRP14B

Intended use

Immunoaffinity columns for use in conjunction with HPLC or LC-MS/MS for detection of ochratoxin A in a wide range of commodities.

General Information

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS.

Benefits:

  • Can be used for the analysis of certain metabolites and masked mycotoxins.
  • The columns give excellent recoveries and low % RSD. Capacity and limit of detection exceed international legislation and CEN / AOAC requirements.
  • The columns are wide format allowing the sample to flow easily by gravity.

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Specifications
Art. NoRBRP14 / RBRP14B
Test format10 columns (3 ml format) (RBRP14),
50 columns (3 ml format) (RBRP14B)
Sample preparationA representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on commodity analysed), filtered and diluted ready for applying to the immunoaffinity column.
LOD (Detection Limit)Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used. Ochratoxin A 0.016 ng/ml
Detected analyte

Ochratoxin A and Ochratoxin B. May also cross react with other metabolites.

Available application notes

Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.

EvaluationHPLC or LC-MS/MS.
ApprovalsThe product has been used in numerous CEN and AOAC collaborative trials and meets all Official Method recovery and capacity specifications.