EuroProxima Plus Bovine Rennet Whey

Art. No.: 5171BRW

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Product info

EuroProxima Plus Bovine Rennet Whey

Art. No.: 5171BRW

Intended use

EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay for screening of the presence of bovine rennet whey in bovine milk and in milk of other species and sources.

General Information

Bovine Rennet Whey (BRW) is a by-product of cheese production from bovine milk. Adulteration of skimmed milk powder with BRW is cost attractive however illegal. Addition of BRW to dairy and other products can be identified by detecting the presence of the bovine glycomacropeptide (GMP), also named casein glycomacropeptide (CMP), which is released from bovine ╬║-casein (kappa-casein) by enzymatic (rennet) hydrolysis during cheese making process and remains as waist product in the curd. EuroProxima Plus Bovine Rennet Whey is a competitive enzyme immunoassay based on antibodies directed against ╬║-casein.
The EuroProxima Plus BRW ELISA detects an epitope located on the glycomacropeptide (CMP) part of ╬║-casein. CMP detected in milk powder is a sign for fraudulent mixing.

 

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Specifications
Art. No5171BRW
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each).
Sample preparationFor mentioned matrices fast and efficient extraction methods are included in the kit description.
Incubation time1 h 30 min (20 - 25 ┬░C)
LOD (Detection Limit)BRW can be detected at a level of 0.1 % in goat's or sheep's milk after a 1:100 dilution of a sample. BRW added to bovine milk or milk powder can be detected down to a level of 1 %.
Validated matrices

Bovine milk/milk powder and milk of other species.

Detected analyte

Glycomacropeptide (CMP) part of ╬║-casein.

EvaluationMicrotiter plate spectrophotometer (450 nm)
Files
InstructionsEnglish
MSDS5171brw.zip (English)