AOF MS-PREP®

Art. No.: RBRP115 / RBRP115B

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Product info

AOF MS-PREP®

Art. No.: RBRP115 / RBRP115B

Intended use

AOF MS-PREP® immunoaffinity columns contain highly specific monoclonal antibodies directed against aflatoxin, ochratoxin A and fumonisin. The columns lead not only to a clean-up of the sample but also to a selective purification of the mycotoxins. Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2 in a wide range of commodities in conjunction with LC-MS/MS.

General Information

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected and injected prior to analysis by LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.

Benefits:

  • One sample preparation and extraction required per sample for the analysis of aflatoxins, ochratoxin and fumonisin, saving time.
  • Simultaneous detection of aflatoxins, ochratoxin and fumonisin in a single LC-MS/MS run.
  • Can be used for the analysis of certain metabolites and masked mycotoxins.

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Specifications
Art. NoRBRP115 / RBRP115B
Test formatRBRP115: 10 immunoaffinity columns
(3 ml format),
RBRP115B: 50 immunoaffinity columns
(3 ml format)
Sample preparationA representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on commodity analysed), filtered and diluted ready for applying to the immunoaffinity column.
LOD (Detection Limit)Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used.

B1 0.007 ng/ml, B2 0.007 ng/ml,
G1 0.004 ng/ml, G2 0.015 ng/ml,
OTA 0.050 ng/ml, FB1 1.000 ng/ml,
FB2 0.250 ng/ml
Detected analyte

Aflatoxin B1. Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Ochratoxin A, Ochratoxin B, Fumonisin B1, Fumonisin B2 and Fumonisin B3. May also cross react with other metabolites and masked mycotoxins.

Available application notes

Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.

EvaluationHPLC or LC-MS/MS.