SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS

Art. No.: S2128

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SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS

Art. No.: S2128

Intended use

The SureFood┬« GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA – sequences.

  • Phosphinothricin-Acetyltransferase gene (BAR) from Streptomyces hygroscopius
  • genetically engineered CryIAb-DNA-sequences and CryIAb/Ac-fusion gene sequences
  • Phosphinothricin-Acetyltransferase gene (PAT) from Streptomyces viridiochromogenes
  • the transition from CTP2 (Chloroplast-Transpeptide-signal sequence from Arabidopsis thaliana) to herbicide tolerance-gene CP4 EPSPS (5-Enolpyruvylshikimat-3-Phosphat Syntheses gene from Agrobacterium tumefaciens strain CP4)

The detections are according to the validated methods of the European Commission or the official collection of detection methods of ┬ž64 German food law.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.

Accessories

Specifications
Art. NoS2128
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended. SureFood® PREP Add On is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
LOD (Detection Limit)The SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS real-time PCR has a limit of detection of < 5 DNA copies. This is equivalent to a limit of detection of <0.01 % based on the genetically modified organisms MON87751 Soya (CryIAb/IAc), DAS-81419-2 Soya (PAT), NK603 Corn (CTP2:CP4 EPSPS) and Bt176 Corn (BAR).
Files
InstructionsGerman/English