SureFood® GMO QUANT Bt11 Corn

Art. No.: S2016

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Product info

SureFood® GMO QUANT Bt11 Corn

Art. No.: S2016

Intended use

The test detects the relative quantitative Bt11 Corn DNA amount.

Therefore the kit contains two PCR systems, one specific for the event Bt11 Corn (OECD unique identifier SYN-BT├ś11-1) and the other one specific for Corn (the reference gene). The event specific Bt11 Corn detection is according to the official method of the European Commission.

 

General Information

The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).

Specifications
Art. NoS2016
Test format2 x 50 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
LOD (Detection Limit)The Bt11 Corn PCR has a limit of detection of ÔëĄ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of corn specific reference gene are present, the relative quantitation limit for Bt11 Corn DNA is 0.1 %.
Files
InstructionsGerman/English