RIDASCREEN® 17ß-Östradiol is a competitive enzyme immunoassay for the quantitative analysis of 17ß-estradiol in bovine plasma.
17β-estradiol is a natural sexual hormone. The levels of 17β-estradiol in bovine plasma from peripheral blood are very low. In calves not more than 1 – 2 pg/ml (ppt) are present and in cycling females the concentration of 17β-estradiol is 1 – 5 pg/ml. Only in plasma of pregnant animals or in plasma of animals illegally treated with anabolic agents, levels in the range of 100 to 1000 pg/ml can be found.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Extraction, freezing and evaporation.|
|Incubation time||2 h 30 min|
|LOD (Detection limit)||Approx. 20 ng/l (ppt)|
|Detected analyte||17ß-estradiol in bovine plasma.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|