PremiĀ®Test 25

Art. No.: R3925

  1. Home
  2. /
  3. Products
  4. /
  5. PremiĀ®Test 25


Product info

PremiĀ®Test 25

Art. No.: R3925

Intended use

PremiĀ®Test is a microbial screening test that allows the detection of antibiotic residues, especially in fresh meat (beef, pork, poultry) in less than 4 hours. The test is sensitive to antibiotics of the following groups: Ī²-lactams, cephalosporins, macrolides, tetracyclines, sulfonamides, aminoglycosides, quinolones, amphenicols and polypeptides.

PremiĀ®Test is a ā€˜Performance Tested Methodā€™ of the AOAC Research Institute for the screening of Penicillin in bovine meat (Certificate No. 060601). Furthermore, it holds the ā€˜VALIDATION NFā€™ by AFNOR for the screening of Ī²-lactams, macrolides, tetracyclines, sulphonamides in beef, pork and poultry (RBP 31/02 ā€“ 04/11).

General Information

Treatment of farm animals with veterinary pharmaceuticals such as antibiotics leads to drug residues in the tissues of the animals and establishes a health risk for the consumers of animal food products. PremiĀ®Test is an easy antibiotics residue screening test which allows fast screening of food products by all members of the food production chain such as farmers, slaughterhouses, meat-processors and analytical laboratories.

PremiĀ®Test is based on the inhibition of the growth of Geobacillus stearothermopilus, a thermophilic bacterium which is very sensitive to many antibiotics and sulfur compounds. A standardised number of spores are imbedded in agar medium containing selected nutrients. When PremiĀ®Test is heated at 64 Ā°C, the spores will germinate. If no inhibitory substances are present the germinated spores will multiply with the production of acid. This will be visible by a color change from purple to yellow. When anti-microbial compounds are present in sufficient amount (above the detection limit) the spores will be unable to germinate and therefore no color change will be observed.



  • Easy to use qualitative screening test for most common antibiotics found in food products as Ɵ-lactams, sulfonamides, macrolides, tetracyclines, macrolides and other antibiotic classes
  • Only minor investment in equipment or laboratory space needed: incubator or water bath and meat press
  • Detection Limits (LOD) are for most parameters below the European Maximum Residue Lewel – MRL


Art. NoR3925
Test format25 ampoules
Sample preparationRecommendations for sample-preparations of fish, shrimps, eggs, kidney, liver,and feed are available at R-Biopharm AG. These matrices require individual validation by the costumer. Supportive information are available at R-Biopharm.

Incubation timeapprox. 3 hours
LOD (Detection Limit)In line with EU Maximum Residue Limits (MRLā€™s).
Detection CapabilityPenicillin G 6 Ī¼g/kg (ppb), amoxicillin 11 Ī¼g/kg, cloxacillin 150 Ī¼g/kg, chlortetracycline 160 Ī¼g/kg, oxytetracycline 160 Ī¼g/kg, doxycycline 100 Ī¼g/kg, sulfadimethoxine 75 Ī¼g/kg, sulfadiazine 90 Ī¼g/kg, erythromycine A 200 Ī¼g/kg, tylosine A 90 Ī¼g/kg
Validated matrices

Meat (beef, pork, poultry)

Detected analyte

Residues of Ī²-lactams, cephalosporines, macrolides, tetracyclines, sulfonamides, aminoglycosides, quinolones, amphenicoles and polypeptides in meat (beef, pork, poultry).