Art. No.: RBRP124 / RBRP124B

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Art. No.: RBRP124 / RBRP124B

Intended use

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxin M1 in milk and dairy products.

General Information

The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. The total extraction and clean-up time takes approximately 30 minutes to perform. The result is improved clean-up and concentration of the toxin from food and feed samples giving a much cleaner chromatogram and therefore providing more accurate and sensitive detection. The columns also have the added advantage that they can be automated for large scale analysis of samples.


  • AFLAPREP┬« M WIDE is an improvement on AFLAPREP┬« M as the wide column is easier to handle and prevents column blockage with high fat content samples.
  • AFLAPREP┬« M WIDE is available in 10 and 50 column kits to suit customers with various testing requirements.
  • Additional Application Notes are available for more complex dairy products.



Art. NoRBRP124 / RBRP124B
Test format10 columns (3 ml format) (RBRP124),
50 columns (3 ml format) (RBRP124B)
Sample preparationA representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on commodity analysed), filtered and diluted ready for applying to the immunoaffinity column.
LOD (Detection Limit)Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used.
M1 0.0039 ng/ml
Detected analyte

Aflatoxin M1 and Aflatoxin M2. May also cross react with other metabolites

Available application notes

Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.

EvaluationHPLC or LC-MS/MS.
ApprovalsThe product meets all Official Method recovery and capacity specifications.