PremiĀ®Test 4×25

Art. No.: R3900

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Product info

PremiĀ®Test 4×25

Art. No.: R3900

Intended use

PremiĀ®Test is a microbial screening test that allows the detection of antibiotic residues, especially in fresh meat (beef, pork, poultry) in less than 4 hours. The test is sensitive to antibiotics of the following groups: Ī²-lactams, cephalosporins, macrolides, tetracyclines, sulfonamides, aminoglycosides, quinolones, amphenicols and polypeptides.

PremiĀ®Test is a ā€˜Performance Tested Methodā€™ of the AOAC Research Institute for the screening of Penicillin in bovine meat (Certificate No. 060601). Furthermore, it holds the ā€˜VALIDATION NFā€™ by AFNOR for the screening of Ī²-lactams, macrolides, tetracyclines, sulphonamides in beef, pork and poultry (RBP 31/02 ā€“ 04/11).


  • Easy to use qualitative screening test for most common antibiotics found in food products as Ɵ-lactams, sulfonamides, macrolides, tetracyclines, macrolides and other antibiotic classes.
  • Only minor investment in equipment or laboratory space needed: incubator or water bath and meat press.
  • Detection Limits (LOD) are for most parameters below the European Maximum Residue Limit (MRL).

General Information

Treatment of farm animals with veterinary pharmaceuticals such as antibiotics leads to drug residues in the tissues of the animals and establishes a health risk for the consumers of animal food products. PremiĀ®Test is an easy antibiotics residue screening test which allows fast screening of food products by all members of the food production chain such as farmers, slaughterhouses, meat-processors and analytical laboratories.

PremiĀ®Test is based on the inhibition of the growth of Geobacillus stearothermopilus, a thermophilic bacterium which is very sensitive to many antibiotics and sulfur compounds. A standardised number of spores are imbedded in agar medium containing selected nutrients. When PremiĀ®Test is heated at 64 Ā°C, the spores will germinate. If no inhibitory substances are present the germinated spores will multiply with the production of acid. This will be visible by a color change from purple to yellow. When anti-microbial compounds are present in sufficient amount (above the detection limit) the spores will be unable to germinate and therefore no color change will be observed.


Art. NoR3900
Test format4 x 25 ampoules
Sample preparationRecommendations for sample-preparations of fish, shrimps, eggs, kidney, liver, and feed are available at R-Biopharm AG. These matrices require individual validation by the costumer. Supportive information are available at R-Biopharm.
Incubation timeapprox. 3 hours
LOD (Detection Limit)In line with EU Maximum Residue Limits (MRLā€™s).
Detection CapabilityPenicillin G 6 Ī¼g/kg (ppb), amoxicillin 11 Ī¼g/kg, cloxacillin 150 Ī¼g/kg, chlortetracycline 160 Ī¼g/kg, oxytetracycline 160 Ī¼g/kg, doxycycline 100 Ī¼g/kg, sulfadimethoxine 75 Ī¼g/kg, sulfadiazine 90 Ī¼g/kg, erythromycine A 200 Ī¼g/kg, tylosine A 90 Ī¼g/kg
Validated matrices

Meat (beef, pork, poultry)

Detected analyte

Residues of Ī²-lactams, cephalosporines, macrolides, tetracyclines, sulfonamides, aminoglycosides, quinolones, amphenicoles and polypeptides in meat (beef, pork, poultry).