SureFast® Listeria 3plex ONE
Art. No.: F5217
The SureFast® Listeria 3plex ONE can be applied for the fast and simple isolation and differentiation of Listeria spp. and Listeria monocytogenes in different types of food.
The method consists of the following three steps:
1) Cultural enrichment
2) DNA-preparation (10 min lysis)
3) specific real-time PCR detection
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels ROX, Cy5 and VIC/HEX at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER and Agilent AriaDx.
|Test format||100 reactions|
|LOD (Detection Limit)||The SureFast® Listeria 3plex ONE real-time PCR has a limit of detection of < 5 DNA copies.|
The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.