RIDASCREEN® Streptomycin

Art. No.: R3104

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Product info

RIDASCREEN® Streptomycin

Art. No.: R3104

Intended use

RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.

General Information

Besides β-lactam antibiotics in veterinary medicine streptomycin is one of the mostly used antibiotics for the treatment of mastitis. Residues of streptomycin may therefore occur in food of animal origin, if the withholding period is not obeyed or if it is used improperly. High concentrations of streptomycin show ototoxic and nephrotoxic effects. The chronic exposure of humans with low concentrations, as found in food, may cause allergies, impair the intestinal flora and induce resistance of pathogenic microorganisms. To protect the consumer against health risks and avoid food-technological problems, a sensitive and simple method for the detection of streptomycin is necessary. The EU regulations for streptomycin are fixed in MRLs (maximum residue limits) for meat and milk (muscle and liver: 500 μg/kg (ppb), kidney: 1000 μg/kg and milk: 200 μg/l). In honey, the EU Community Reference Laboratories have determined a zero-tolerance with a minimum required performance limit of ccβ = 40 μg/kg for test systems.

Benefits:

  • Broad range of matrices: milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.
  • Improved sensitivity.
  • Short test: 45min.
Specifications
Art. NoR3104
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each).
Sample preparationMilk: dilution, milk powder: reconstitution, dilution

Honey: extraction, chromatographic clean-up with RIDA® C18 column, evaporation, reconstitution

Meat, liver, kidney, shrimp: homogenization, extraction, dilution

Apple juice: centrifugation, dilution
Incubation time45 min
LOD (Detection Limit)Milk approx. 5 ÎĽg/l (ppb), milk powder (reconstituted from milk powder) approx. 3 ÎĽg/kg, honey approx. 2 ÎĽg /kg, beff, pork approx. 22 ÎĽg/kg, poultry approx. 28 ÎĽg/kg, liver approx. 23 ÎĽg/kg, kidney approx. 18 ÎĽg/kg, shrimp approx. 20 ÎĽg/kg, apple juice approx. 4 ÎĽg/l
Detection CapabilityMilk 10 µg/l, milk powder µg/kg, honey 5 µg/kg, beef, pork 50 µg/kg, poultry 50 µg/kg, liver 50 µg/kg, kidney 50 µg/kg, shrimp 50 µg/kg, apple juice 10 µg/l
Validated matrices

Milk, milk powder, honey, meat, liver, kidney, shrimp and apple juice.

Detected analyte

Streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.

EvaluationMicrotiter plate spectrophotometer (450 nm)