Art. No.: RBRDP126 / RBRP126
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of citrinin in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS.
- The immunoaffinity columns give excellent recoveries and low % RSD. High capacity and low limit of detection.
- The columns are wide format allowing the sample to flow easily by gravity.
- Improved clean-up and concentration of toxin from complex food matrices resulting in reduced chromatography interference and lower detection limits.
|Art. No||RBRDP126 / RBRP126|
|Test format||10 columns (3 ml format) (RBRDP126),|
25 columns (3 ml format) (RBRP126)
|Sample preparation||A representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on commodity analysed), filtered and diluted ready for applying to the immunoaffinity column.|
|LOD (Detection Limit)||Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used. |
Citrinin 2.34 ng/ml (ppt)
Citrinin. May also cross react with other metabolites.
|Available application notes|
Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.
|Evaluation||HPLC or LC-MS/MS.|