AFLAPREP®
Art. No.: RBRDP07 / RBRP07
Product info
Intended use
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform.
General Information
The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when anal HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.
Benefits:
- The columns give excellent recoveries and low % RSD. Capacity and limit of detection exceed international legislation and CEN / AOAC requirements.
- Improved clean-up and concentration of toxin from complex food matrices resulting in reduced chromatography interference and lower detection limits.
- The columns are suitable for testing a wide range of commodities.
Accessories
| Specifications | |
|---|---|
| Art. No | RBRDP07 / RBRP07 |
| Test format | 10 columns (1 ml format) (RBRDP07), 50 columns (1 ml format) (RBRP07) |
| Sample preparation | A representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on the commodity analysed) filtered and diluted ready for applying to the immunoaffinity column. |
| LOD (Detection Limit) | Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used. B1 0.031 ng/ml B2 0.016 ng/ml G1 0.031 ng/ml G2 0.004 ng/ml |
| Detected analyte | Aflatoxin B1, Aflatoxin B2, Aflatoxin G1 and Aflatoxin G2. May also cross react with other metabolites. |
| Available application notes | Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information. |
| Evaluation | HPLC or LC-MS/MS. |
| Approvals | The product has been used in numerous CEN and AOAC collaborative trials and meets all Official Method recovery and capacity specifications. |
