AFLAOCHRA PREP®

Art. No.: RBRP89 / RBRP89B

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Product info

AFLAOCHRA PREP®

Art. No.: RBRP89 / RBRP89B

Intended use

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2 and ochratoxin A in a wide range of commodities in conjunction with HPLC or LC-MS/MS.

General Information

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected and injected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.

Benefits:

  • The columns have been validated and approved by NestlĂ©.
  • One sample preparation and extraction for the analysis of aflatoxins and ochratoxin, saving time.
  • Simultaneous detection of aflatoxins and ochratoxin in a single HPLC run. Alternatively, analysis can be performed in a single LC-MS/MS run.

Accessories

Specifications
Art. NoRBRP89 / RBRP89B
Test formatRBRP89: 10 immunoaffinity columns
(1 ml format),
RBRP89B: 50 immunoaffinity columns
(1 ml format)
Sample preparationA representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on commodity analysed), filtered and diluted ready for applying to the immunoaffinity column.
LOD (Detection Limit)Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used.

HPLC
B1 0.0079 ng/ml, B2 0.0079 ng/ml, G1 0.0157 ng/ml, G2 0.0079 ng/ml, OTA 0.0125 ng/ml.
LC-MS/MS
B1 0.007 ng/ml, B2 0.007 ng/ml, G1 0.004 ng/ml, G2 0.015 ng/ml, OTA 0.050 ng/ml
Detected analyte

Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2 and Ochratoxin A and Ochratoxin B. May also cross react with other metabolites.

Available application notes

Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.

EvaluationHPLC or LC-MS/MS.
ApprovalsThe product meets all Official Method recovery and capacity specifications.