Solid phase clean-up columns for the purification of aflatoxins B1, B2, G1 and G2 from peanuts.
PuriTox Aflatoxin can be used for the clean-up of peanut samples by HPLC or LC-MS/MS. In addition, the columns can be used for the clean-up of pigmented samples before ELISA to reduce background interference. The clean-up columns can be used in conjunction with HPLC or LC-MS/MS for the analysis of peanut samples. The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.
- One sample preparation and extraction required per sample for the analysis of aflatoxin and zearalenone, saving time and money.
- Optimised HPLC methods for the analysis of aflatoxin in peanuts (PuriTox Aflatoxin) or aflatoxin and zearalenone in cereals (PuriTox AflaZON).
- Easy-to-use-syringe format suitable for testing peanuts or cereals – can be used with a vacuum manifold if required.
|Test format||50 columns (syringe format)|
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (5 - 50 g dependent on method used) of his is removed and extracted.|
|Incubation time||60 sec|
|LOD (Detection Limit)||B1 0.08 ng/ml (ppt), B2 0.08 ng/ml, |
G1 0.20 ng/ml, G2 0.08 ng/ml
Aflatoxins B1, B2, G1 and G2 in peanuts.
|Evaluation||It is recommended to run at least a 3 - 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|