Intended use:

RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour).

General Information:

The use of wheat flour and gluten in foodstuffs is extremely common because of their heat stability and useful effects on e.g. texture, moisture retention and flavour. Gluten is a mixture of prolamin and glutelin proteins present in wheat, rye and barley. Coeliac disease is a permanent intolerance to gluten that results in damage to the small intestine and is reversible when gluten is avoided by diet. According to the Codex Alimentarius (CODEX STAN 118/1979) two categories for labeling of food according to the gluten content now exist:
1.) “Glutenfree” = Food products, containing < 20 mg / kg Gluten
2.) “Very low gluten” = Food products containing 20 – 100 mg / kg Gluten
The threshold of 20 mg / kg has been adopted by many national legislations in many countries.

The sample preparation using the Cocktail (patented) (R7006 / R7016) is the official R5-Mendez method according to the Codex Alimentarius and the AOAC. The faster sample preparation using the environmental-friendly Cocktail ECO (R7080) is convenient for the screening of samples. The Cocktail ECO has an extraction efficiency of approx. 70 – 110 % compared to Cocktail (patented).


  • Based on the R5 antibody plus further antibodies against other gluten proteins.
  • Different sample extractions validated depending on the Matrices (Cocktail patented (R7016), Cocktail ECO (R7080)) adapted to the specific requirements of the different samples.
  • Fast and environmental-friendly Cocktail ECO (R7080) reducing sample preparation from 120 min (Cocktail patented (R7016)) to 35 min.


Article NumbersR7051
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation Homogenization and extraction
Incubation time 30 min
Room temperature
LOD (Detection limit) 0.2 mg / kg (ppm) Gliadin (mean) or to 0.4 mg / kg (ppm) Gluten
0.19 – 0.21 mg / kg (ppm) Gliadin*
*depending on matrix
LOQ (Limit of quantification)

1.25 mg / kg (ppm) Gliadin or 2.5 mg / kg (ppm) Gluten

Validated matrices

Gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour).

Available application notes
  • Sample preparation for processed food with the RIDA® Extraction Solution (colorless) (R7098) – only after validation.
  • Sample preparation for raw materials with ethanol.
  • Sample preparation for polyphenol containing raw materials (e.g. chocolate, cofee, cacao, buckwheat) with fish gelatine and ethanol.
Detected analyte
  • Prolamins from wheat, rye and barley (spelt, kamut)
  • Epitopes which are related to celiac disease (beside others one peptide sequence in the α-gliadin)
  • ω-gliadin
Evaluation Microtiter plate spectrophotometer (450 nm)
Similar products
RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin ...
RIDASCREEN® FAST Gliadin is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by prolamins from wheat (gliadin), rye (s...
RIDASCREEN® Gliadin competitive
RIDASCREEN® Gliadin competi...
RIDASCREEN® Gliadin competitive is used for the analysis of fermented and hydrolyzed food (e.g. beer, starch syrup, starch, malt extract, sourdough...

Product support

Questions? Tap into our team’s expertise. We’re here to support you and your business throughout the testing process to ensure your success.

Start typing and press Enter to search