Genetically modified organisms

Innovative solutions for the detection of GMO in food

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Analytes

Genetically modified organisms

The world wide production of genetically modified food (e.g. soy and corn) has steadily increased since the beginning of the 90ÔÇÖs.

Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labelled. As stated by the Council of Ministers of the European Union (EU), regulation for the labelling of Novel Food: Decision No. 1139/98/EC. Furthermore, since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0.9 % in EU for food and feed.

Labelling of GMO-free food

The quality of food is becoming increasingly important to many consumers. To ensure the necessary transparency, i.a. the European Council Regulation (EC) (No. 834/2007) on organic production was implemented. This regulation regulates the use and labeling of genetically modified plants in food and feed (European labeling requirements 1829/2003 and 1830/2003). Compliance with this basic organic regulation can be shown by a corresponding seal on the food products.

GMO analysis according to VLOG

Further information about the VLOG requirements and the recommended analytics can be found in our flyer.

Qualitative GMO detection

According to national or regional regulations, zero tolerance applies to non-approved GMO events. As such, only qualitative tests are of interest for these products at the moment. The detection limit lies at 0.01 %, depending on the matrix and the application.

Regulation EC 619/2011 exists as a special rule for animal feeds. Provided that certain conditions are met (expired EU approval, approval in the country of origin, availability of tests and reference material, etc.), a technical threshold value of 0.1 % applies for non-approved GMO events. This is particularly significant for not always contamination-free imports of GMOs and GMO-free maize and soya.

Quantitative GMO detection

For respectively approved GMO events, there is the quantitative question of whether the product lies above the defined threshold values or mandatory labelling values.

In Europe, a value of 0.9 % applies to the GMO event in the respective plant matrix. Below that value, foodstuffs with technically unavoidable and coincidental contaminations of the GMO events do not need to be declared (EC 2829/2003 und EC2830/EC). The quantification occurs via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.

GMO screening

A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides efficient evidence of the presence of any possible GMOs.

The typically used gene sequences are:

This promoter, made from the cauliflower mosaic virus, is the most frequently used element. Plant samples that are only 35S positive can also be contaminated with the naturally occurring virus. False positive results can be ruled out by using the virus-specific CaMV (S2027) test.

The Nopalin Synthase Terminator (NOS) from Agrobacterium tumefaciens is often but not exclusively used in combination with 35S.

In lieu of 35S, the figwort mosaic virus promoter can also be used in GMOs.

The gene for phosphinotricin-N-acetyltransferase from Streptomyces hygroscopicus can enable a resistance against the herbicide glufosinate that is available under several different brand names.

This sequence denotes the transition from chloroplast transit peptide to 5-enolpyruvylshikimate-3-phosphate synthase from Arabidopsis thaliana and Agrobacterium spp. It enables a resistance against the herbicide glyphosate.

These and further parameters can either be combined in a modular way or used individually. The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (S2049).

SureFood® is a modular system which is based on the 3 main components , amplification and detection. Each component consists of different modules. All modules are compatible. Thus, SureFood® is a highly flexible system which can be easily configured for different user specific applications. SureFood® is available as product and service as well.

SureFood® GMO product overview

You can find a comprehensive overview of our SureFood® GMO test kits in our free brochure.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
TANBead MaelstromÔäó 4800

The TANBead MaelstromÔäó 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

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Up to 48 samples ZMAL48
SureFood® GMO QUANT MON810 Corn

The test detects the relative quantitative MON810 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON810 Corn (OECD unique identifier MON-├ś├ś81├ś-6) and the other one specific for Corn (the reference gene). The …

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2 x 50 reactions S2019
SureFood® GMO ID DAS-40278-9 Corn

The SureFood┬« GMO ID DAS-40278-9 Corn is a real-time PCR kit for the direct, qualitative detection of a specific genetically modified corn DNA sequence. Therefore the kit contains an event specific real-time PCR system for the DAS-40278-9 corn (OECD …

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100 reactions S2140
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead┬« Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of …

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96 preparations F1060
TANBead MaelstromÔäó 8 Autostage

The TANBead MaelstromÔäó 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing …

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Up to 8 samples ZMAL8
SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS

The SureFood┬« GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA – sequences. Phosphinothricin-Acetyltransferase gene …

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S2128
SureFood® GMO ID 4plex Corn I

The SureFood┬« GMO ID 4plex Corn I is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified corn DNA sequences: ┬áFAM channel: MON810 corn (OECD unique identifier MON-├ś├ś81├ś-6) VIC/HEX …

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100 reactions S2170
SureFood® GMO ID 4plex Soya II

SureFood┬« GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modified soya DNA sequences listed as below: Roundup Ready Soya (RR Soya) (OECD unique identifier …

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100 reactions S2162
SureFood® GMO QUANT MON89034 Corn

The test detects the relative quantitative MON89034 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON89034 Corn (OECD unique identifier MON-89├ś34-3) and the other one specific for Corn (reference system). The …

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2 x 50 reactions S2071
SureFood® GMO QUANT TC1507 Corn

The test detects the relative quantitative TC1507-Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the TC1507-Corn (OECD unique identifier DAS-├ś15├ś7-1) and the other one specific for Corn (reference system). The …

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2x 50 reactions S2081
SureFood® GMO ID 4plex Canola II

The multiplex test detects the following DNA-sequences of genetically modified canola: – FAM channel: MON88302 canola (OECD unique identifier MON-883├ś2-9) – VIC channel: Canola – ROX channel: DP73496 canola (OECD unique identifier …

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100 reactions S2167
SureFood® GMO ID 4plex Canola I

The multiplex test detects the following DNA sequences of genetically modified canola: FAM channel: MS8 canola (OECD unique identifier ACS-BN├ś├ś5-8) ROX channel: GT73 canola (OECD unique identifier MON-├ś├ś├ś73-7) Cy5 channel: T45 canola (OECD …

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100 reactions S2166
SureFood® GMO ID 4plex Soya I

The multiplex test SureFood┬« GMO ID 4plex Soya I detects the following DNA-sequences of soya: FAM-channel: MON87708 Soja (OECD Bezeichnung MON-877├ś8-9) FAM-channel: CV127 Soja (OECD Bezeichnung BPS-CV127-9) VIC-channel: DP305423 Soja (OECD …

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100 reactions S2161
SureFood® GMO ID CV127 Soya (R&D Version)

Detection of CV 127 Soya.

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100 reactions S2066
SureFood® GMO ID DP305423 Soya (R&D Version)

The test detects DP305423 Soya (OECD unique identifier DP-3├ś5423-1) DNA. The event specific detection is according to the official method of the European Commission. The real-time PCR assay can be used with established real-time PCR instruments …

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100 reactions S2065
SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS

This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. The multiplex test detects the following DNA-sequences: Phosphinothricin-Acetyltransferase gene (BAR) from Streptomyces hygroscopius …

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100 reactions S2127
SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC

The SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC is a real-time PCR for the direct, qualitative detection and differentiation of following specific DNA sequences.

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100 reactions S2158
SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton

The SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton is a real-time PCR for the direct, qualitative detection and differentiation of following specific corn/soya/canola/cotton DNA sequences.

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100 reactions S2156
SureFood® GMO QUANT GT73 Canola

The test detects the relative quantitative GT73 Canola DNA amount. Therefore the kit contains two PCR systems, one specific for the GT73 Canola (OECD unique identifier MON-├ś├ś├ś73-7) and the other one specific for Brassicacea family (reference …

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2 x 50 reactions S2061
SureFood® GMO QUANT NK603 Corn

The test detects the relative NK603 Corn DNA amount to the total corn DNA content. Therefore the kit contains two PCR systems, one for detection of a NK603 Corn specific gene (OECD unique identifier MON-├ś├ś6├ś3-6) and one for the detection of a corn …

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2×50 reacions S2050
SureFood® GMO QUANT MON863 Corn

The test detects the relative quantitative MON863 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON863 Corn (OECD unique identifier MON-├ś├ś863-5) and the other one specific for corn (the reference gene). The event …

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2 x 50 reactions S2051
SureFood┬« GMO ID A2704-12 Soya – Discontinued in 2021 – Replaced by S2161

The test detects A2704-12 Soya DNA. Therefore the kit contains an event specific real-time PCR system for the A2704-12 Soya (OECD unique identifier ACS-GM├ś├ś5-3). The event specific detection is according to the official method of the European …

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100 reactions S2057
SureFood® GMO QUANT GA21 Corn

The test detects the relative quantitative GA21-Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the GA21-Corn (OECD unique identifier MON-├ś├ś├ś21-9) and the other one specific for Corn (reference system). The …

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2 x 50 reactions S2054
SureFood® GMO PLANT PLUS

The test detects DNA of plants qualitatively. Each reaction contains an internal amplification control (IAC).    

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100 reactions S2049
SureFood® GMO QUANT MIR162 Corn

The test detects the relative quantitative MIR162-Corn DNA amount. The kit contains two PCR systems, one event-specific for the MIR162-Corn (OECD unique identifier SYN-IR162-4) and the other one specific for Corn (reference system).  

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2 x 50 Reactions S2135
SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC

This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. For this purpose, PCR systems for detection of the 35S Cauliflower Mosaic Virus (CaMV) promoter DNA sequence, A. tumefaciens NOS terminator DNA …

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100 reactions S2126
SureFood® GMO SCREEN CaMV

The test detects CaMV DNA. The presence of CaMV DNA indicates an infestation with the cauliflower mosaic virus. Host of the CaMV are Brassicaceae plants. With the virus specific detection system SureFood┬« GMO CaMV it is possible to verify the …

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100 reactions S2027
SureFood┬« GMO SCREEN 35S + NOS + FMV – Discontinued in 2021 – Replaced by S2126

This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. For this purpose, PCR systems for detection of the 35S CAMV promoter DNA sequence, A. tumefaciens NOS terminator DNA sequence and 34S FMV promoter …

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100 reactions S2026
SureFood® GMO QUANT T25 Corn

The test detects the relative quantitative T25-Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the T25-Corn (OECD unique identifier ACS-ZM├ś├ś3-2) and the other one specific for Corn (reference system). The …

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100 reactions S2017
SureFood® GMO QUANT RR2Y Soya

The test detects the relative quantitative RoundupReady2 Yield-Soya DNA amount (RR2Yield-Soya). Therefore the kit contains two PCR systems, one specific for the RR2Yield-Soya (OECD unique identifier MON-89788-1) and the other one specific for Soya …

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2×50 reactions S2029
SureFood® GMO QUANT RoundUp Ready Soya

The test detects the relative quantitative Roundup Ready Soya DNA amount (RR-Soya). Therefore the kit contains two PCR systems, one specific for the Roundup Ready Soya (OECD unique identifier MON-├ś4├ś32-6) and the other one specific for Soya …

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2×50 reactions S2014
SureFood® GMO QUANT MON 810 Corn

The test detects the relative quantitative MON810 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON810 Corn (OECD unique identifier MON-├ś├ś81├ś-6) and the other one specific for Corn (the reference gene). The …

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2 x 50 reactions S2019
SureFood® GMO QUANT Bt176 Corn

The test detects the relative quantitative Bt176-Corn DNA amount. Therefore the kit contains two PCR systems, one construct specific for the Bt176-Corn (OECD unique identifier SYN-EV176-9) and the other one specific for Corn (reference system). …

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100 reactions S2015
SureFood® GMO QUANT Bt11 Corn

The test detects the relative quantitative Bt11 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the event Bt11 Corn (OECD unique identifier SYN-BT├ś11-1) and the other one specific for Corn (the reference gene). The …

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2 x 50 reactions S2016
SureFood® GMO QUANT 35S Soya

The test is developed for the relative estimation of 35S CaMV promoter DNA amount in only Soya containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Soya (the …

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2×50 reactions S2028
SureFood® GMO QUANT 35S Corn

The test is developed for the relative estimation of the 35S CaMV DNA amount in only Corn containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Corn (the …

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2×50 reactions, whereof 50 reactions are used for the quantification of the reference gene. S2020
SureFood® GMO SCREEN P35S:BAR Rice

The test detects the genetically construct P35S:BAR. The LibertyLink 601 (LL601 – OECD unique identifier BCS-OS├ś├ś3-7) Rice and the LibertyLink 62 (LL62 – OECD unique identifier ACS-OS├ś├ś2-5) Rice contains this genetically construct but …

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100 reactions, whereas 50 reactions are used for detection of the reference gene. S2022
SureFood® GMO ID Bt63 Rice

The test detects Bt63 Rice DNA. The detection of Bt63 Rice is a construct-specific real-time PCR assay. Additionally the SureFood┬« GMO ID Bt63 Rice kit contains a reference-PCR system for the detection of rice. The real time PCR system used in the …

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100 reactions S2024
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